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Tumor cytogenetics

Chromosome analysis is now part of the standard diagnostics of hematological tumor disease. Somatic changes are determined in the tumor DNA. They provide an overview of the respective karyotype. The karyotype is necessary for the diagnosis, classification of disease and has usually prognostic and therapeutic significance.

Fluorescence in situ hybridization (FISH) is mainly used in addition to chromosome analysis and is needed to answer specific questions (e.g. for rapid targeted diagnosis within 4 hours and for the detailed characterisation of chromosomal abnormalities). The FISH technique has the advantage that not only metaphase cells (cells undergoing nuclear division), but also interphase nuclei (cells not undergoing division) can be investigated. In many cases, the response to therapy can be determined by FISH.

The average processing time of a chromosomal analysis of bone marrow and tumor tissue is 11 days.

 

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